Integrated transcriptomics, proteomics and metabolomics-based analysis uncover TAM2-associated glycolysis and pyruvate metabolic remodeling in pancreatic cancer

Introduction Tumor-associated macrophage 2 (TAM2) abundantly infiltrates pancreatic ductal adenocarcinoma (PAAD), and its interaction with malignant cells is involved in the regulation of tumor metabolism. In this study, we explored metabolic heterogeneity TAM2 by constructing TAM2-associated subtypes PAAD. Materials methods PAAD samples were classified into molecular different characteristics based on a multi-omics analysis strategy. 20 tissues 10 normal collected for proteomic metabolomic analyses. RNA sequencing data from TCGA-PAAD cohort used transcriptomic Immunohistochemistry was to assess infiltration tissues. Results The results transcriptomics immunohistochemistry showed that levels upregulated associated poor patient prognosis. proteomics metabolomics indicated multiple processes aberrantly regulated dysregulation linked level infiltration. WGCNA confirmed pyruvate glycolysis/gluconeogenesis as co-expressed pathways Based data, four (quiescent, pyruvate, mixed). Metabolic each characterized terms clinical prognosis, microenvironment, immune cell infiltration, chemotherapeutic drug sensitivity, functional mechanisms. Conclusion Our study remodeling closely related TAM2. Molecular provided new insights prognosis prediction therapy patients.